TRANSCRIPTOME SEQUENCING OF LEPISANTHES FRUTICOSA TO DISCOVER SSR MARKERS

Lepisanthes fruticosa (ceri Terengganu) is one of the important underutilized fruit plants with high value bioactive compounds and pharmacological properties. Current studies have focused mainly on which are essential for functional food pharmaceutical applications. However, diversity conservation L. still scarce since genomic genetic resources this plant species lacking. In study, RNA sequencing leaf was carried out using Illumina HiSeq to identify potential unigenes simple sequence repeats (SSRs). A total 52,657 were identified from about 91,043,356 million raw reads. Mining SSRs these predicted a 23,958 approximately 45.58% obtained. Dinucleotide motif highest (21.48%) next trinucleotide (14.65%). 4,620 ranging 12 116 bp selected experimental validation. Bioinformatic analysis via GO KEGG platforms showed that 1,861 (40.28%) SSR-containing matched Gene Ontology (GO) terminology 48 biochemical pathways. The involved in various cell functions majority their associated purine thiamine metabolism. addition. organic heterocylic bindings, indicating an active event biosynthesis process secondary metabolites fruticosa. SSR markers obtained study provides new information can be utilized facilitate future characterization accessions at molecular levels.